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Supplementary Figure 2 – Neural patterning and morphogenesis are disrupted by translation-blocking sall morpholinos Translation-blocking morpholinos induce changes in neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 (A-L) and 23 (M-O) using markers otx2, krox20, and hoxB9 in combination (A-C), pax3 (D-F), pax6 (G-I), sox2 (J-L), and tubb2b (M-O); dorsal view, anterior up. Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Compare to Figure 2, splice-blocking morpholinos; see Figure 2 for quantification of neural marker phenotypes in all sall morphants. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 16 anterior egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 16 rhombomere R3 rhombomere R5 hoxb9.L hox 2.5, xhoxb9, XlHbox-6, XlHbox6 X. laevis Throughout NF stage 16 posterior pax3.L cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 16 neural plate neural plate border pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 16 neural plate anterior neural fold pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 16 neural plate tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 23 neural tube Image source: Published Larger Image Printer Friendly View

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