Xenbase Image ID: 157823

Supplementary Figure 4 – Neural crest marker gene expression, but not epidermal gene expression, is reduced in sall morphants sall1 and sall4 morpholinos do not affect epidermal markers (A-H) but do induce changes in neural crest marker gene expression (I-L), as assayed by whole-mount RNA in situ hybridization at stage 16; dorsal view, anterior up. Splice-blocking sall1 and sall4 morpholinos were injected unilaterally into the embryos shown here. Representative images are shown for epidermal keratin (epi ker, A-D), gata2 (E-H), and snai2 (I-L). Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Values in the lower right of each panel indicate the number of embryos with the depicted expression pattern. Expression of snai2 in sall morphants at earlier stages is shown in Supplementary Figure 5. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 16 non-neural ectoderm gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 16 non-neural ectoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 16 cranial neural crest Image source: Published Larger Image Printer Friendly View

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