Xenbase Image ID: 157826

Supplementary Figure 7 – Apical actin accumulation is disrupted by translation-blocking morpholinos Confocal microscopy after transverse sectioning and staining of morphants; dorsal is up. Sections were taken from the anterior third of embryos, where primary neurulation is most easily observed. F-actin (phalloidin, green), microtubules (anti-DM1α antibody, red), nuclei (DAPI, blue). Asterisks in images indicate injected side; white arrows indicate loss of actin accumulation. A’-C’ show higher magnification views of A-C. Scale bars = 50μm. s = somite, noto = notochord. Compare to Figure 3 F-I’, splice-blocking morpholinos; see Figure 3 J for quantification of phenotypes in all sall morphants. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue actb.L beta actin, beta-actin, g-actin X. laevis Throughout NF stage 16 neural plate tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 16 neural plate Image source: Published Larger Image Printer Friendly View

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