Xenbase Image ID: 157846

Fig. 2 (left). FoxD1 neural patterning activity is modified via canonical wnt signaling. (A) Embryos were injected animally at the one-cell stage with mRNAs encoding foxd1 (50 pg) or dkk1 (25 pg) proteins. AC explants were removed from control and injected embryos at blastula-stage and grown to neurula stage 17. Total RNA was isolated from five control embryos (lane 2) and eighteen ACs from each group (lanes 3-6). Various neural AP markers were examined by sqRT-PCR: anterior forebrain and cement gland (xanf1, xag1, otx2), posterior hindbrain, spinal cord and primary neuron markers (krox20, hoxb9, ntub) and panneural markers (nrp1, ncam). In the different samples, ef1a serves as a control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1). (B) Embryos were injected animally at the one-cell stage with mRNA encoding foxd1 (20 pg) protein or a CMV-driven plasmid vector (20 pg) driving wnt3a expression. ACs were removed from control and injected embryos at blastula-stage and grown to neurula stage 17. Total RNA was isolated from five control embryos (lane 2) and eighteen ACs from each group (lanes 3-6). Various neural AP markers were examined by sqRT-PCR: anterior forebrain and cement gland (xanf1, xag1, otx2), posterior hindbrain and spinal cord markers (krox20, hoxb9) and panneural markers (nrp1, ncam). In the different samples, ef1a serves as a control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1). Image published in: Polevoy H et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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