Xenbase Image ID: 157847

Fig. 3 (right). FoxD1 neural caudalizing activity is uncoupled from its neural-anteriorizing activity. (A) Embryos were injected animally at the one-cell stage with mRNAs encoding foxd1 (25 pg) or BMP4 (250 pg) proteins. AC explants were removed from control and injected embryos at blastula-stage and grown to late gastrula/early neurula stage. Total RNA was isolated from five control embryos (lane 2) and eighteen ACs from each group (lanes 3-6). Various neural AP markers were examined by sqRT-PCR: anterior forebrain markers (xanf1, otx2,), posterior hindbrain, spinal cord, and primary neuron markers (gbx2, hoxb9, cdx1, ntub) and the panneural marker (ncam). In the different samples, ef1a serves as a control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1). (B) Embryos were injected animally at the one-cell stage with mRNAs encoding foxd1 (25 pg) or BMP4 (250 pg) proteins. AC explants were removed from control and injected embryos at blastula-stage and grown to mid-late neurula stage. Total RNA was isolated from five control embryos (lane 2) and eighteen ACs from each group (lanes 3-6). Various neural AP markers were examined by sqRT-PCR: anterior forebrain and cement gland markers (xanf1, otx2, xag1), posterior spinal cord markers (hoxb9, cdx1, cdx4) and the panneural marker (ncam). In the different samples, ef1a serves as a control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1). Image published in: Polevoy H et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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