Xenbase Image ID: 157850

Fig. 6. FoxD1 and Wnt signaling interact to pattern dorsal mesoderm. (A) Embryos were injected marginally at the one-cell stage with mRNA encoding the foxd1 (25 pg) or dkk1 (40 pg) protein. VMZ explants were removed from control and injected embryos at gastrula stage 10.25, and VMZs were cultured to neurula stage 17. Total RNA was isolated from six control embryos (lane 2), and from eighteen VMZs from each group (lanes 3-6). Expression of the xnot and MA markers was examined by sqRT-PCR. (B) Embryos were injected marginally at the one-cell stage with mRNA encoding the noggin (20 pg) and dkk1 (40 pg) proteins. Embryos were than co-injected with either mRNA encoding the foxd1 (30 pg) protein or the FoxD1-MO (45 pg). VMZ explants were removed from control and injected embryos at gastrula stage 10.25, and VMZs were cultured to neurula stage 17. Total RNA was isolated from six control embryos (lane 2), and from eighteen DLMZs from each group (lanes 3-6). Expression of the anterior (gsc, nkx2.5), dorsal (chd), and dorsal lateral (MA) markers was examined by sqRT-PCR. (C) Embryos were injected marginally at the one-cell stage with mRNA encoding the foxd1 (25 pg) and dkk1 (40 pg). DLMZ explants were removed from control and injected embryos at gastrula stage 10.25, and cultured to neurula stage 17. Total RNA was isolated from six control embryos (lane 2), and from eighteen DLMZs from each group (lanes 3-6). Expression of the anterior (gsc,nkx2.5), dorsal (chd, xnot), and dorsal lateral (myod) markers was examined by sqRT-PCR. (D) Embryos were injected marginally at the one-cell stage with mRNA encoding the noggin (20 pg) and dkk1 (40 pg) proteins. Embryos were than co-injected with either mRNA encoding the foxd1 (30 pg) protein or the FoxD1-MO (45 pg). DLMZ explants were removed from control and injected embryos at gastrula stage 10.25, and DLMZs were cultured to neurula stage 17. Total RNA was isolated from six control embryos (lane 2), and from eighteen DLMZs from each group (lanes 3-7). Expression of the anterior (gsc), dorsal (chd, xnot), and dorsal lateral (MA) markers was examined by sqRT-PCR. Image published in: Polevoy H et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

Return to previous page