Xenbase Image ID: 157986

Supplementary Fig. S2. (A) The sequence design of MO-A and the control 5-base-mispaired MO. The sequences of pseudoalleles a and b are shown below. The red letters stand for the initial Met codon. (B) The sequence design of MO-B and the control 5-base-mispaired MO. (C) Western blot analysis of translates from xDel1 (pseudoallele a; tagged with V5) RNA that contained the 5′ UTR sequence. The translation of xDel1 in the animal cap (stage 9) was specifically inhibited by co-injection of MO-A, but not by that of the control MO. (D) Western blot analysis of translates from xDel1 (pseudoallele b; tagged with V5) RNA that contained the 5′ UTR sequence. The translation of xDel1 was specifically inhibited by co-injection of MO-B, but not by that of the control MO. (E) Percentages of the xDel1 MO-injection phenotypes (suppression of Chd and Gsc expression, and upregulation of Vent1 and Szl expression). Top, MO-A alone; middle, MO-B alone; bottom, both combined. Image published in: Arakawa A et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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