Xenbase Image ID: 157997

Fig. 1. Amino acid sequence alignments of Xenopus Wbp2nl to human WBP2 and WBP2NL. (A)Xenopus Wbp2nl (NP_001088037.1 [laevis] and NP_001107397.1 [tropicalis]) is highly similar to human WBP2 (NP_036610.2). Blue bar indicates the predicted PH-G domain and green bar indicates the predicted WWbp domain. Highly conserved motifs and α-helix are indicated by red bars. Five single amino acid mutations that were experimentally tested for function are indicated by black arrows (T45A, Y55F, Y91G, F127P, Y282F). AP, adaptor protein complex; ER, endoplasmic reticulum retention signal; ITSM, immunoreceptor tyrosine-based switch motif; SH2, Scr Homology domain 2; YAP, Yes associated protein binding motif. (B) Comparison of human WBP2NL (AAH22546.1) to Xenopus sequences shows large regions of disimilarity. Another human WBP2NL entry (Q6ICG8.1) is identical to AAH22546.1 except there is a glutamine at position 285 instead of a histidine. (C) Four examples of cells immunostained for Myc-Wbp2nl (green). Nuclei are stained with DAPI (blue). Note that staining is perinuclear (red arrows), and in cytoplasmic tubular structures (blue arrows) consistent with affiliation with Endoplasmic reticulum. Red asterisks denote intracellular yolk platelets. Image published in: Marchak A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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