Xenbase Image ID: 158011

Figure 2—figure supplement 2 MEK1 is required to inhibit the expression of the pluripotency genes pou5f3.2 and ventx2. (A) Embryos injected with 25 ng Mk-MO at 16-cell stage in one animal dorsal blastomere were grown until late gastrulation stage 13 and processed for WISH with pou5f3.2 and ventx2 probes. (B) Embryos injected with 25 ng Mk-MO at 16 cell stage in one animal ventral blastomere were grown until mid-neurula stage 18 and processed for WISH with pou5f3.2 probe. (C) Four-cell embryos were injected in each blastomere with 25 ng Mk-MO and grown until blastula stage 9, when animal caps were isolated, cultured in vitro until late gastrula stage 13 and then processed for RT-qPCR. In A and B, the number of embryos exemplified by the photograph over the total number of embryos analyzed is indicated. Image published in: Scerbo P et al. (2017) © 2017, Scerbo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue ventx2.L ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. laevis Throughout NF stage 13 non-neural ectoderm pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 13 neural plate notoplate Image source: Published Larger Image Printer Friendly View

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