XB-IMG-158016
Xenbase Image ID: 158016
|
Figure 4—figure supplement 1
Ventx2 knockdown restores germ-layer formation in MEK1-deficient embryos.
(A) Four-cell embryos were injected with 50 pg 2SAVentx2-Myc RNA per cell, fixed at tailbud stage 25 and processed for WISH with pou5f3.2 probe. (B) Four-cell embryos were injected with 30 ng Ventx2-MO (Vx2-MO) per blastomere, collected at stage 10.5 and processed for RT-qPCR. (C) Embryos injected as in B were processed for WISH analysis at early gastrula stage 10.5 with ventx1 and gsc probes (vegetal view). (D). Four-cell embryos were injected with 25 ng Mk-MO, with or without 7.5 ng Vx2-MO, in each blastomere, collected at gastrula stage 10.5, and processed for WISH with indicated probes. Note that embryos stained for xk81a1 (epidermis) were injected in one ventral animal blastomere at 16 cell stage and collected at late gastrula stage 13. Embryos stained for gsc were hemisectioned prior to staining to improve probe penetration. In A and D, the number of embryos exemplified by the photograph over the total number of embryos analyzed is indicated. For the RT-qPCR graph, error bars represent s.e.m. values of three independent experiments with two technical duplicates. For statistical analysis, samples from injected embryos were compared with samples from uninjected control embryos by Unpaired Student’s t-test. *p<0.05, **p<0.005, ***p<0.0001. Image published in: Scerbo P et al. (2017) © 2017, Scerbo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
Image source: Published Larger Image Printer Friendly View |