Xenbase Image ID: 158024

Cells derived from subcutaneous tissues contribute to skin regeneration. A: Illustration of a metamorphosed froglet indicating the transverse planes of sections shown as B-J and as E'-J'. Gray and red rectangles represent the grafted donor skin and excisional skin wound, respectively. B–D: Samples of intact skin regions were sectioned through the plane indicated by the dotted line as “B–D” in A. GFP-positive subcutaneous tissues were observed beneath the GFP-negative grafted skin. E–J: Samples of regenerating skin, including subcutaneous tissues, were sectioned through the plane indicated by the dotted line “E–J” at 2 or 3 weeks after skin excision. E–G: GFP-positive cells had accumulated underneath the GFP-negative wound epidermis at 2 weeks after skin injury (n = 4). Three of four individuals showed basically the same results. H–J: GFP-positive cells contributed to the regenerated skin, especially to the dermis in the center of the wound (n = 4). Four of four individuals showed basically the same results. Asterisks indicate secretion glands. White arrowheads (E–G) indicate the right-hand border of the wound. Pairs of white arrowheads (H–J) indicate the left- and right-hand borders of the wound. Open arrowheads indicate GFP-negative cells contributing to the dermis of the regenerating skin. Bar = 100 µm. Image published in: Otsuka-Yamaguchi R et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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