Xenbase Image ID: 158074

Figure 2 β-Trcp forms a complex with both β-catenin and Skp1. (A) Schematic diagram of wild-type β-Trcp, β-TrcpδF, and β-TrcpδWD. (B) Immunoprecipitation of β-catenin. The precipitates were examined for β-Trcp (Upper) and Skp1 (Lower). A longer exposure revealed that endogenous β-catenin also precipitated β-Trcpmyc (data not shown). (C) Immunoprecipitation of β-Trcpmyc using the same embryo extracts as in B. The precipitates were examined for Skp1 (Upper) and β-Trcp (Lower). (D) Immunoblot of Skp1 in the same embryo extracts as in B. RNA injected per embryo: 1 ng each for β-Trcpmyc and for deletion mutants β-catenin and Skp1. Image published in: Liu C et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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