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Figure 6 Inhibition of endogenous β-Trcp function by β-TrcpδF stabilizes β-catenin in vivo. Accumulation of β-cateninFlag or β-catenin (S→A)Flag protein was examined in stage 9 embryo extracts by immunoblotting with an anti-Flag antibody. Immunoblotting of tubulin was used as a protein loading control (Lower). This experiment were performed twice with similar results. RNA injected per embryo: 50 pg for β-cateninFlag or β-catenin (S→A)Flag; 4 ng for β-Trcp or β-TrcpδF. Image published in: Liu C et al. (1999) Copyright © 1999. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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