Xenbase Image ID: 158170

Figure 4. Flow cytometer setup to detect the three fluors in the new reporter assay. A: Gate setup was arranged to minimize spectral overlap and background. For eGFP in FL1 for cells transfected with the IRES-GFP, control vector relegates GFP counts to the right of the vertical line in quadrant G4. B: Cells transfected with CMV-HcRed control plasmid, recorded in FL5 and gated for HcRed1 expression, relegate HcRed counts to above the horizontal line in quadrant G1. C: Gated population of 10,000 cells expressing eGFP, HcRed1, and DsRed (Ds Red, indicative of promoter activity, is counted in quadrant G2). D: Histogram of DsRed output in FL2 for the population of eGFP and HcRed co-expressing cells. Image published in: Hooker LN et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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