Xenbase Image ID: 158183

Fig. 4. Overexpression of five out of six selected vegetally enriched mRNAs reduces PGC number. One-cell embryos were injected in the vegetal region with GFP (control) or the indicated transcripts (0.5 ng). Tailbud embryos (stage 32-35) were analyzed for xpat expression by WISH, and representative images are shown (A-G). The number of PGCs per embryo was quantified (H). GFP, n=16; spire1, n=39; e2f1, n=64; otx1, n=27; parn, n=35; rras2, n=48; wwtr1, n=35. *P<0.05, compared with GFP control. Analysis based on at least two independent experiments and shown as a box and whisker plot. Image published in: Owens DA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pgat.L Xpat X. laevis Throughout NF stage 32 primordial germ cell Image source: Published Larger Image Printer Friendly View

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