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XB-IMG-158184

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Fig. 5. MO-mediated knockdown of a subset of vegetally enriched mRNAs increases PGC number. One-cell embryos were injected in the vegetal region with MOs (15 ng) targeting otx1 or wwtr1. Tailbud embryos (stage 32-35) were analyzed for xpat. Representative images are shown (A-C). The number of PGCs per embryo was quantified (D). Uninjected control (ctrl), n=32; otx1-MO, n=31; wwtr1-MO, n=25. *P<0.05 compared with control. Analysis based on at least two independent experiments.

Image published in: Owens DA et al. (2017)

Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
pgat.LXpatX. laevisThroughout NF stage 32primordial germ cell

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