XB-IMG-158186
Xenbase Image ID: 158186
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Fig. 7.
efnb1 is required for normal PGC number and migration. One-cell embryos were injected in the vegetal region with (A) efnb1-FL alone (200 pg), or efnb1-FL (200 pg) and efnb1-MO (16 ng); (B) scrambled-MO (16 ng), efnb1-MO alone (16 ng), or efnb1-MO (16 ng) and efnb1-FL-rescue (200 pg). Tailbud embryos were analyzed for xpat expression by WISH. Representative images are shown on the left. (A) The number of PGCs per embryo was quantified. (B) Percentage of embryos with mislocalized PGCs was calculated. Black lines indicate the boundaries of normal PGC location, between somites 5 and 11. Uninjected control (Ctrl), n=28; efnb1-FL, n=28; efnb1-FL and efnb1-MO, n=28; scrambled-MO, n=25; efnb1-MO, n=27; efnb1-MO and efnb1-FL-rescue, n=28. *P<0.05 compared with uninjected control. #P<0.05 compared with efnb1-FL (A) or efnb1-MO (B). Analysis based on at least two independent experiments. Image published in: Owens DA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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