XB-IMG-158190
Xenbase Image ID: 158190
|
Figure S3. Morpholino-mediated sox7 inhibition reduces PGC number in tailbud embryos.
A) Schematic of the sox7 morpholino target region (top). Wheat germ extract was incubated with
sox7-FL (500ng) in the presence of increasing concentrations of sox7-MO and subject to anti-flag
western blot analysis. Quantification of respective flag expression is shown (bottom). B-D) Onecell
embryos were injected in the vegetal region with sox7-MO (16ng). Tailbud embryos were
analyzed for xpat expression by WISH. The number of PGCs per embryo was quantified (B).
Representative images are shown (C-D). Uninjected control (ctrl) n=36, sox7-MO n=43. *
statistically significant compared to ctrl (p<0.05). Analysis based on at least two independent
experiments. Image published in: Owens DA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published Larger Image Printer Friendly View |