Xenbase Image ID: 158204

Fig. 3. Loss of neural crest derivatives in Apoc1 depleted embryos. (A) The Apoc1 translational blocking MO (tbMO) targets the 5’ UTR region of the Xenopus laevis apoc1 gene. To determine the efficiency of the tbMO, two flag-tagged apoc1 constructs were used differing only in the region upstream of the ATG start site. (B) Western blot using an anti-flag antibody on lysates from embryos injected with 300 pg 5’UTR-apoc1-flag or apoc1-flag mRNA and 40 ng Apoc1 tbMO or 40 ng control MO (ctrl). (C) 30 ng of the splice blocking morpholino (spMO) or control MO (ctrl) was injected into the animal side of two blastomeres at the two-cell stage and the embryos were assayed by RT-PCR at stage 10.5. Odc is used as loading control. (D) Schematic drawing of the experiment. 40 ng Apoc1 MO was injected into either the presumptive head (F,G) or trunk/tail (H,I) region together with 200 pg lacZ mRNA. (E) Embryos injected with 40 ng control MO were normal. (F,G) Injections targeted to the head region resulted in 76.1% (n=134) of the embryos displaying small or missing eyes and head deformation. (H,I) Injections targeted to the trunk region resulted in perturbed dorsal fin development in 55.2% (n=38) of the embryos. The pictures in (G,I) were taken prior to X-gal staining (F,H). Image published in: Yokota C et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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