Xenbase Image ID: 158205

Fig. 4. Apoc1 is required for neural border formation and neural crest emergence. (A) Whole mount in situ hybridization for the neural crest (NC) markers c-myc, sox9, snai2, twist1, id3. Control MO injected embryos are shown in upper panel and Apoc1 tbMO injected ones are shown in lower panel. (B) The reduction of sox9 and snai2 expression by the Apoc1 tbMO could be rescued by coinjection of 400 pg of apoc1 mRNA (upper panel) but not by coinjection of 400 pg of sp-apoc1 mRNA (lower panel). (C) Whole mount in situ hybridization for the neural plate border specifiers msx1, pax3, zic1, the neural marker sox2, and the non-neural ectoderm marker xk81a1. Control MO injected embryos are shown in upper panel and Apoc1 tbMO injected ones are shown in lower panel. One of the dorsal blastomeres was injected with 30 ng of either control MO (upper panels in A, C) or Apoc1 tbMO (lower panels in A, C) together with 100 pg lacZ mRNA. Embryos were fixed at stage 15. The injected side was traced by X-gal staining (blue). Image published in: Yokota C et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue myc c-myc, cmyc, myc-a, myc-b, X-cmyc, Xcmyc X. tropicalis Throughout NF stage 18 cranial neural crest sox9 cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. tropicalis Throughout NF stage 15 cranial neural crest snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 15 cranial neural crest twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. tropicalis Throughout NF stage 15 cranial neural crest id3 heir-1, id3-a, id3-b, xid3, xidia, xIdx X. tropicalis Throughout NF stage 15 neural plate pre-chordal neural plate chordal neural plate msx1 hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. tropicalis Throughout NF stage 15 neural plate border pax3 cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. tropicalis Throughout NF stage 15 neural plate border zic1 odd-paired-like, opl, Zic-r1, Zic-related-1 X. tropicalis Throughout NF stage 15 neural plate border sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. tropicalis Throughout NF stage 15 neural plate krt12.4 ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. tropicalis Throughout NF stage 15 non-neural ectoderm Image source: Published Larger Image Printer Friendly View

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