Xenbase Image ID: 158206

Fig. 5. Apoc1 can substitute for wnt in the animal cap neural crest induction assay. (A) 200 pg of chrd, 200 pg wnt3a and/or 300 pg of apoc1 was injected into two animal blastomeres of 4- or 8-cell stage embryos. Animal caps were dissected at stage 9 and cultured until stage 19. Gene expression was assayed by RT-PCR. (B) 125 pg tBR (dominant negative form of bmpr1a) and 30 pg aN-HA-Ctnnb1 (constitutively active form of ctnnb1) mRNA was injected in two animal blastomeres at the 4- to 8-cell stage to induce neural crest cell marker expression. In addition, 40 ng of either control (ctrl) or translation blocking Apoc1 MO (tbMO) with or without 400 pg MO-resistant apoc1 mRNA was injected to determine if Apoc1 is required for neural crest induction. Animal caps were dissected at stage 9 and cultured until stage 16. Gene expression was assayed by RT-PCR. (C) Coinjection of apoc1 and zic1 into the ventral side of the embryo induced ectopic expression of sox9 and snai2. 50 pg of zic1 +/- 500 pg of apoc1 was injected into ventral marginal cells of 8 cell-stage embryos. The embryos were fixed at stage 14, and subjected to whole mount in situ hybridization. The blue arrows point to endogenous sox9 and snai2 expression. The yellow arrowheads show the area of ectopic sox9 and snai2 expression. Image published in: Yokota C et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue sox9 cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. tropicalis Throughout NF stage 15 cranial neural crest snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 15 cranial neural crest Image source: Published Larger Image Printer Friendly View

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