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Fig. S3. Apoc1 mutant lacking signal sequence cannot be secreted. (A) Schematic drawing of the Apoc1 secretion experiment. mRNA was injected at the two-cell stage. The blastopore fluid was collected at the gastrula stage and subjected to western blot (WB) analysis to determine whether mutant flag-tagged Apoc1 protein (lacking signal sequence) is secreted from cells during embryonic development. (B) Apoc1, but not mutant protein was detected by WB in the blastocoel fluid after injection of 500pg of flag-tagged apoc1 or flag-tagged mutant apoc1 mRNA. The tubulin blot shows that there is no cellular contamination in the blastopore fluid. Image published in: Yokota C et al. (2017) Copyright © 2017. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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