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Fig 4. Rescuing the morpholino phenotype. Embryos were injected with 100ng morpholino and 5ng cRNA of the various constructs. Embryos were allowed to develop to stage 38. The experiments were repeated in triplicate and the results were combined, n values shown at top of graph. (A) schematic of rescue constructs. (B) Embryos scored for pigmentation effect as scale in Fig 2. NI non injected, WT wild type, AT A111T, DN D383N, XL X.laevis, mo morpholino, CMO control morpholino. Kruskal Wallis test revealed significant difference between 100ng ATG MO and the WT, AT, DN and XL constructs, p = 0.001 for all, but not 4C p = 0.988. https://doi.org/10.1371/journal.pone.0180465.g004 Image published in: Williams RM et al. (2017) © 2017 Williams et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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