Xenbase Image ID: 158330

Figure 1 Endogenous Genes Show Regulation Not Captured by the WRE (A) In the canonical Wnt pathway, Wnt ligand stimulation inhibits the destruction complex, resulting in the accumulation of β-catenin. Together with Tcf/Lef proteins, β-catenin binds to the WRE and activates or represses target genes. (B) Xenopus embryos were treated with LiCl for 5 min at the 32-cell stage, harvested at stage 10 for qRT-PCR assay, and scored 3–4 days later (shown here). (C) Expression of target genes, siamois (black circle) and Xnr3 (white circle). Control embryos are untreated sibling embryos. Red arrows highlight how gene expression remains wild-type despite perturbations. (D) Black circle, luciferase/renilla signal from the TopFlash reporter injected at the four-cell stage; white circle, β-catenin level in the embryo measured using western blot. Red arrows highlight how β-catenin level and TopFlash expression change with moderate perturbations. (B)–(D) are reproduced from [5] with permission. Data are represented as mean ± SEM from three to five biological replicates. Error bars not visible have negligible SEM. Image published in: Kim K et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page