Xenbase Image ID: 158437

Fig. 1. Transcriptional targets of Sox17β. Embryos were injected at the two-cell stage with increasing doses mRNA of encoding Sox17β (62 pg, 125 pg, 250 pg, 500 pg), animal cap explants were isolated at blastula (stage 9), cultured until gastrula (stage 11) and assayed for endodermal gene expression by RT-PCR. None of the endodermal genes was expressed in uninjected animal cap tissue (–), but all were expressed in gastrula whole embryos. Ef1α, is a loading control; –RT, without reverse transcriptase; +RT, with reverse transcriptase. Image published in: Sinner D et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + sox17b.1 + animal cap explant + NF11 (RT-PCR) fig.1 Expression Phenotype increased amount a2m.L expression in animal cap increased amount foxa1.L expression in animal cap increased amount foxa2.L expression in animal cap increased amount gata4.L expression in animal cap increased amount gata5.L expression in animal cap increased amount gata6.L expression in animal cap increased amount gsc.L expression in animal cap increased amount hao1.L expression in animal cap increased amount hnf1b.L expression in animal cap increased amount nodal.L expression in animal cap increased amount sox17a.L expression in animal cap increased amount sox17b.2.L expression in animal cap Larger Image Printer Friendly View

Return to previous page