Xenbase Image ID: 158439

Fig. 5. The transactivation domain of Sox17β is required for β-catenin binding. COS-1 cells were transfected with (A) 3 μg of DNA encoding the indicated V5-epitope tagged Sox17β constructs or (B) 3 μg of DNA encoding the indicated V5-epitope tagged Sox17α constructs. The resulting cell extracts were either incubated with GST-agarose or GST-β-catenin-agarose. The input and bound V5-tagged proteins were visualized by anti-V5 immunoblotting. Mutations or deletion of the conserved transactivation motif in either Sox17β or Sox17α impairs or abolishes β-catenin binding. Image published in: Sinner D et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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