XB-IMG-158442
Xenbase Image ID: 158442
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Fig. 8. β-catenin is required for the expression of Sox17 targets genes at gastrula stage. (A) Two-cell embryos were vegetally injected with either 20 ng of antisense β-catenin morpholino oligos (MO) and/or 100 pg of RNA encoding a N-terminal deleted form of stabilized β-catenin (δN-β-cat) (Yost et al., 1996), which does not contain the sequence targeted by the antisense oligo. At a series of stages throughout gastrulation (stages 10, 11 and 12) whole embryos were harvested and assayed by real-time RT-PCR for the expression of Sox17 target genes as well as several other control genes. The histograms show the relative expression levels normalized to the loading control, ODC. For simplicity only the stage 11 data (stage 10 for Xnr1, Xnr2, Xnr4 and Derriere) is shown. Edd, Hnf1β, Foxa1 and Foxa2 are direct Sox17 target genes. Siamois, Hex and Cerberus are known β-catenin target genes. Xnr1, Xnr2, Xnr4, Derreire and Mixer are endodermal genes that are not Sox17 targets. Plakoglobin (Plako) and Ef1α are control genes that are neither Sox17 nor β-catenin targets. (B) A proportion of each sample from the same experiment was assayed by immunoblotting with either anti-β-catenin or anti-tubulin antibodies. Injected δN-β-catenin protein has a higher molecular weight than endogenous β-catenin because of the presence of an epitope tag. Image published in: Sinner D et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |