XB-IMG-158443
Xenbase Image ID: 158443
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Figure 1. Microinjection Analysis of the CAC-Rich Regions of 3′ UTRs from Six Localized mRNAs
(A) Various fragments from the 3′ UTRs of six different localized RNAs were injected into stage I oocytes and assayed for localization to the mitochondrial cloud. White arrows indicate where RNA (blue) has localized to the mitochondrial cloud in all panels except for XβG (Xenopus β-globin), which was used as a negative control for localization and shows no labeling of the mitochondrial cloud. The numbers in the lower right corner of each panel indicate the number of oocytes that showed localization over the number of oocytes tested from a minimum of three independent experiments.
(B) A schematic representation of all six 3′ UTRs is shown, with the name of each construct that was assayed for localization shown at the right and preceded by a letter indicating its corresponding panel in (A). Regions containing the CAC-rich clusters are indicated by open lines with coordinates from the experimental LE column in Table 1. A scale bar in nucleotides is included at the bottom of the figure. Note that all CAC-rich clusters show localization and that removal of the CAC-rich region from the Xpat 3′ UTR (c) or deletion of the UGCAC motifs from Xcat-2 LE (e) abolishes localization.
(C) A schematic diagram of the Macho-1 mRNA is shown at the left, with the sequence of the CAC-rich region of its 3′ UTR from Table 1 shown in the box. All CAC and ACAC motifs are shown in red text. The CA of each motif was mutated to GU, indicated below the sequence of each CAC or ACAC motif. It can be seen that the wild-type (wt), but not the mutant (mut) version of the Macho-1 CAC-rich region localizes in Xenopus stage I oocytes. Image published in: Betley JN et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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