Xenbase Image ID: 158549

Fig. 6. Model simulations of cell sorting and tissue separation. Simulations of sorting from a mixed aggregate (a, c) and of maintenance of a boundary interface (b, d). a, b Representative snapshots at the indicated number of iterations. Rectangular images represent enlargements of a portion of the aggregates. Initial matrices are shown at 0 iteration. c, d Evolution of LHI used as an index for cell sorting and for maintenance of separation. Small panels show details of the first 1000 iterations. The curves represent the average of 15, respectively, 8, independent simulations. Error bars: s.d. In the case of mixed aggregates, each simulation started from a different initial matrix of randomly distributed cells. The same sets of relative contact energies were used for both types of situations. DITH scenarios differ in terms of the relative heterotypic interfacial energy, set at 25, 50, 75 and 100% of the difference between the homotypic tension energies of the two cell populations. DITH* corresponds to the previously published conditions37; HIT conditions were set with identical homotypic energies in both cell types, and a higher interfacial tension; ectoderm–mesoderm (E–M) energies were based on estimated relative tensions in the Xenopus system. Cell to medium values were set as follows: a, b 25 for both cell types, all conditions. In graphs c and d, unequal values 18/9 for all conditions except for the two HIT conditions, where they were set equal, respectively, 9/9 and 18/18. Varying cell to medium values had little impact on sorting and separation (see systematic comparisons in Supplementary Fig. 8b–g) Image published in: Canty L et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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