Xenbase Image ID: 158558

Supplementary Figure 1 | Characterization of induced mesoderm. (a) Characterization of dissected tissues by RT-PCR. Cerberus is a marker for anterior mesendoderm, Gsc, and Chordin for prechordal mesoderm, Xbra for chordal mesoderm, Sox17 is enriched in the endoderm, but also expressed at lower levels in the mesoderm. Ubiquitously expressed ODC was used as a standard. Quantification is from 3 independent experiments. Error bars are s.d. (b) C-cadherin (red) and β-catenin (green) immunostaining in the gastrula embryo. Nuclei were stained with Hoechst (blue). BCR (Ecto), ectoderm blastocoel roof; AME, anterior mesendoderm; AxM, axial mesoderm. Arrowheads point to the ectoderm-mesoderm boundary. The axial mesoderm is still mostly at the blastopore lip, but starts to participate to the nascent boundary (red arrowhead) and already displays separation activity from ectoderm in vitro 1. Image published in: Canty L et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 18 to NF stage 22 gastrocoel roof plate endomesoderm axial mesoderm cdh3.L b-cadherin, c-cadherin, cdh3-a, cdh3-b, cdhc-A, cdhp, EP-cadherin, hjmd, P-cadherin, pcad, U-cadherin, xb-cad, XB-cadherin, XBcad X. laevis Sometime during NF stage 18 to NF stage 22 axial mesoderm gastrocoel roof plate endomesoderm Image source: Published Larger Image Printer Friendly View

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