Xenbase Image ID: 158599

Fig. 3. Anterior neural tube morphogenesis requires thehmmrmicrotubule binding domains. (A) Schematic representation of hmmr full length (FL) and deletion (δ) constructs lacking amino- (δN) or carboxy-terminal (δC) amino acids (AA) or both (δN+C). MTBD, microtubule binding domain. (B, C) Control embryo (B) and embryo injected unilaterally with hmmr morpholino (MO) into the right dorsal animal blastomere at the eight cell stage (st.; injected side marked by asterisk; C) and bisected transversally at the forebrain level at st. 21, views onto cut surfaces. Dashed black lines mark border of neural tissue and indicate the midline. Wildtype neural tissue width depicted by green arrow, widened neural tissue by red arrow. (D) Statistical analysis of neural tissue width upon injection of hmmr MO and deletion constructs. Note that presence of the MTBDs is required for rescue of tissue narrowing. Number of embryos indicated for each plot. ns, not significant. Image published in: Prager A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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