Xenbase Image ID: 158602

Fig. 6. hmmr and vangl2 interact during anterior neural morphogenesis. Reciprocal rescue of anterior neural morphogenesis and cell polarity defects in hmmr and vangl2 morphants. Embryos at the eight cell stage (st.) were injected into the right dorsal animal blastomere with morpholinos (MO) and mRNA as indicated and analyzed at st. 21. (A) Anterior neural morphogenesis. Statistical analysis of neural tissue width (cf. Fig. 3). ns, not significant; number of embryos analyzed indicated for each plot. Note that hmmr and vangl2 interaction in mediating neural tissue narrowing requires the N-terminal microtubule (MT) binding domains of hmmr. (B-E) Neural cell polarity. Comparison of cell shape (indicated with white dashed lines) and MT organization (Tuba4a, red). Insets demonstrate lineage tracer fluorescence in the respective area. Frequency of phenotypes indicated in each panel. Note that despite the failure to rescue tissue narrowing (A), hmmr δN re-establishes cell polarization (E). Image published in: Prager A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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