Xenbase Image ID: 158607

Fig. S4. Posterior neural tube morphogenesis inhmmrmorphants. (A, A’) Embryo injected unilaterally with hmmr morpholino (MO) into the right dorsal animal blastomere at the eight cell stage (st.) and bisected transversally through the posterior neural plate at st. 21 as indicated in inset. View onto cut surface, lineage tracer (LT) fluorescence marks targeted tissue (A). Dashed white lines mark border of neural tissue and indicate midline (A’). Wildtype (WT) and widened neural tissue indicated by green and red arrow, respectively. (B) Statistical analysis of neural tissue width in control and treated embryos, number of embryos indicated for each plot. (C, D) Comparison of cell shape (indicated by white dashed lines) and microtubule organization (Tuba4a, red) in WT (C) and morphant (D). Frequency of phenotypes indicated in each panel; inset shows LT fluorescence in the respective area. Image published in: Prager A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tubal3.L tuba1 X. laevis Throughout NF stage 21 microtubule neural plate Image source: Published Larger Image Printer Friendly View

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