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Supplementary Figure 1. GS17 in situ hybridization in embryos with altered nuclear size and ploidy See Figure 2 legend for experimental approach. (a) GS17 staining intensity was quantified in both halves of 7 hpf diploid and haploid control embryos, and normalized to the diploid control. N = number of embryos. Error bars represent SD. * p < 0.05. (b) GS17 staining intensity was quantified in pre-MBT diploid and haploid embryo halves with increased nuclear size, and normalized to the diploid with increased nuclear size. N = number of embryos. Error bars represent SD. * p < 0.05. (c) Haploid embryos were microinjected with mRNA to increase nuclear size. Cells in 4.5 hpf embryos that received the microinjected mRNA were identified by rhodamine dextran fluorescence, and nuclear size was quantified for GS17-positive and GS17- negative cells. The bottom panels show representative images of two cells that received the microinjected mRNA and showed differential GS17 staining and nuclear sizes. N = number of cells. Error bars represent SD. *** p <0.001. Scale bar, 20 μm. (d) Diploid and haploid embryos were microinjected with mRNA to increase nuclear size. Cells in 4.5 hpf embryos that were positive for GS17 staining and exhibited similar nuclear sizes were analyzed. The top graph shows average nuclear sizes, and the bottom graph shows average GS17 staining intensity for those same cells, normalized to the diploid. N = number of cells. Error bars represent SD. * p < 0.05; NS = not significant. (e) Comparisons were performed between diploid control embryos and haploid embryos microinjected with mRNA to increase nuclear size. Cells in 7 hpf embryos that were positive for GS17 staining and exhibited similar nuclear sizes were analyzed. The graph on the left shows average nuclear sizes, and the graph on the right shows average GS17 staining intensity for those same cells, normalized to the diploid control. N = number of cells. Error bars represent SD. * p < 0.05; NS = not significant. Image published in: Jevtić P and Levy DL (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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