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Fig. S1. Sox and Tcf target specificity. RNA encoding the indicated Sox or Tcf (200 pg) was injected into the animal region of two-cell embryos. Animal caps were isolated at stage 9 and assayed at stage 11-12 by real-time RT-PCR. The results are normalized and shown as a ratio to ODC. This experiments was repeated three times and a representative is shown. With the exception that Sox3 and Lef induced Gata6 and Gsc, none of the other HMG box factors reproducibly induced significant expression of Sox17 targets. Image published in: Sinner D et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + gsc + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount gata6.L expression in whole organism increased amount gsc.L expression in whole organism Xla Wt + lef1 + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount gata6.L expression in whole organism increased amount gsc.L expression in whole organism Xla Wt + sox11 + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount gata5.L expression in whole organism increased amount nodal.L expression in whole organism Xla Wt + sox17a + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount a2m.L expression in whole organism increased amount foxa1.L expression in whole organism increased amount foxa2.L expression in whole organism increased amount gata4.L expression in whole organism increased amount gata5.L expression in whole organism increased amount gata6.L expression in whole organism increased amount gsc.L expression in whole organism increased amount hao1.L expression in whole organism increased amount hnf1b.L expression in whole organism increased amount nodal.L expression in whole organism Xla Wt + sox17b.1 + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount a2m.L expression in whole organism increased amount foxa1.L expression in whole organism increased amount foxa2.L expression in whole organism increased amount gata4.L expression in whole organism increased amount gata5.L expression in whole organism increased amount gata6.L expression in whole organism increased amount gsc.L expression in whole organism increased amount hao1.L expression in whole organism increased amount hnf1b.L expression in whole organism increased amount nodal.L expression in whole organism Xla Wt + sox3 + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount hao1.L expression in whole organism increased amount hnf1b.L expression in whole organism Xla Wt + tcf3 + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount gata6.L expression in whole organism Xla Wt + tcf4 + NF11-12 (RT-PCR) fig.S1 Expression Phenotype increased amount a2m.L expression in whole organism increased amount foxa1.L expression in whole organism increased amount gata5.L expression in whole organism increased amount gata6.L expression in whole organism Larger Image Printer Friendly View

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