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Fig. S2. Tcf3 does not regulate Sox17 target genes in vivo. Embryos were injected at the two-cell stage with antisense morpholino oligos to b-catenin (20 ng) or Tcf3 (20 ng), as previously described (Heasman et al., 2000; Houston et al., 2002). At gastrula stage, five embryos from each condition were pooled and assayed by real-time RT-PCR and relative gene expression was normalized and presented as a ratio to ODC. As a control, the expression of the ubiquitously expressed gene plakoglobin was unchanged. As expected, Hex was downregulated by b-catenin depletion, but upregulated by Tcf3 depletion. This is the predicted result observed from all Tcf3-regulated genes (Houston et al., 2002) and is consistent with de-repressing Tcf3 activity. By contrast, the Sox17 target genes Hnf1b, Edd and Foxa2 were not upregulated in Tcf3-depleted embryos but were repressed in b-catenin depleted embryos. This indicates that they are not Tcf3 targets but are b-catenin targets. Consistent with the results presented in the paper, Foxa1 was not significantly regulated by b-catenin levels. Image published in: Sinner D et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + ctnnb1 MO + gastrula stage (RT-PCR) fig.S2 Expression Phenotype decreased amount a2m.L expression in whole organism decreased amount foxa2.L expression in whole organism decreased amount hhex.L expression in whole organism decreased amount hnf1b.L expression in whole organism Xla Wt + tcf3 MO + gastrula stage (RT-PCR) fig.S2 Expression Phenotype increased amount hhex.L expression in whole organism Larger Image Printer Friendly View

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