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Fig. S3. Direct Sox17b targets. This experiment is a repeat of that presented in Fig. 2B, but analyzed by real time RT-PCR to better show the quantitation of the induced transcription. Isolated blastula animal cap explants either injected with mRNA encoding GR:Sox17b (150 pg) or uninjected were each cultured in three conditions; 1xMBS, 1xMBS + 10^-6 dexamethasone (DEX) or in 1xMBS with 10^-6 dexamethasone + 10 mg/ml cycloheximide (CHX). Dexamethasone activates the GR:Sox17b fusion protein and cycloheximide blocks translation. Control animal caps were treated with 5 ng/ml human activin A either with or without 10 mg/ml cycloheximide. At gastrula (stage 11), the explants were assayed by RT-PCR. Image published in: Sinner D et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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