XB-IMG-158693
Xenbase Image ID: 158693
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Fig. 1. Analysis of gene expression patterns in the developing
pronephros by whole-mount single-probe in situ hybridization. A
developmental series of embryos was hybridized with Xwnt-4 (A-C),
Xlim-1 (D-F), xWT-1 (G-I), and XPax-2 (J-L). In all cases, anterior is to
the right and dorsal is up. Stages shown are early tailbud (stage 23, A,
D, G, J), mid- tailbud (stage 26, B, E, H, K), and tadpole (stage 33, C, F,
I, J). Note that Xwnt-4 is expressed only in the anteriodorsalmost cells
of the anlage by stage 23. This expression corresponds to the distal tip
of the tubules at later stages (arrow in C). Xlim-1 is initially expressed
throughout the pronephric anlage including the pronephric tubules
and duct (arrowhead in F) and refines to the distal tips at late tadpole
stages (arrow in F). xWT-1 is expressed in a similar pattern to that of
Xwnt-4 at early tailbud stages. However, the two patterns are
drastically different at later stages (compare staining marked by
arrow in C-I). XPax-2 is expressed at low levels in the pronephric
anlage at early tailbud stages and maintained in the tubules and duct
(arrowhead in L) through later stages of development with strongest
expression in the distal portion of the three tubules (arrow in L). Image published in: Carroll TJ et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |