XB-IMG-158694
Xenbase Image ID: 158694
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Fig. 2. Comparison of expression patterns. Gene expression patterns
were directly compared through whole-mount and histological
examination of singly and doubly probed in situ hybridized embryos.
In frames A-I, dorsal is at top and lateral is at left. For reference, the
lateral margin of the endoderm is indicated by a black line in panels A
and E. A-D: Transverse sections through the pronephric anlage of
stage 22 embryos singly probed with Xwnt-4 (A), xWT-1 (B), Xlim-1
(C), or XPax-2 (D). E: Transverse sections through the pronephric
anlage of a stage 26 embryo doubly probed with Xwnt-4 (blue) and
xWT-1 (pink). e’: A more anterior transverse section through the same
embryo as shown in E. Note the overlap of expression domains of
Xwnt-4 and xWT-1 (arrow, purple staining). F-H: Transverse sections
of pronephric anlagen at stage 28. f’-h’: Anterior sections through the
same embryos. F, f’: Double probe with Xwnt-4 (blue) and xWT-1
(pink). G, g’: Double probe with Xlim-1 (blue) and xWT-1 (pink). H, h’:
Double probe with XPax-2 (purple) and xWT-1 (pink). I: Transverse
section through the pronephros at stage 30 doubly probed with XPax-2
(blue) and xWT-1 (pink) showing that the expression patterns are
entirely mutually exclusive at this stage. J: Lateral view of a
whole-mount stage 26 embryo probed with XPax-2 and Xlim-1. The
purple represents overlap of expression of the two genes within the
duct (blue arrow) and the tubules (black arrow). The light blue (red
arrow) represents cells that are expressing only XPax-2. Image published in: Carroll TJ et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |