XB-IMG-158746
Xenbase Image ID: 158746
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Figure 5. Onset of the MBT in the presence of the cleavage Inhibitor Cytochalasin B.
Eggs (50) were transferred 40 min after fertilization into 0.5 ml MMR
containing 5 fig/ml cytochalasin B and 0.5 mCi of either ‘H-thymidine
or 3H-uridine. Control eggs were incubated in the absence of cyto- chalasin B. Samples were removed at the indicated times and pre- pared for either autoradiography (RNA synthesis) or TCA precipitation (DNA synthesis). (open circle) DNA synthesis in controls; (solid circle) DNA synthesis in blastomeres treated with cytochalasin B. RNA synthesis was quanti- tated as described in Figure 28. (open square) RNA synthesis in controls: (solid square) RNA synthesis in blastomeres treated with cytochalasin B. Image published in: Newport J and Kirschner M (1982) Copyright © 1982. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |