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Figure 8. Premature Onset of Transcription ir. Polyspermic Eggs Polyspermic fertilization was performed by the method of Grey et al. (1982). The number of sperm nuclei per egg was determined by counting the number of pigmented sperm entry points. Eggs were blocked in cleavage by centrifugation and were injected with 1 &I w3’P-rUTP 60 min after fertilization. At various times eggs were collected and analyzed for RNA transcripts as described in Figure 4. (Lanes a-e) Polyspermic eggs; (lanes f-j) normal eggs. Times after fertilization were as follows: 5 hr (lanes a and f); 6 hr (lanes b and g); 7 hr (lanes c and h); 8 hr (lanes d and i); 9 hr (lanes e and j). Image published in: Newport J and Kirschner M (1982) Copyright © 1982. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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