Xenbase Image ID: 158800

Fig. S2. (A) Chip used to hold the embryos in place for time-lapse imaging and/or repeated exposure to light. The trap (Adams et al. 2014; Zhu et al. 2014) is made of PDMS and has been filled with blue dye to show the shape of the microfluidics chamber. White arrows indicate the direction of flow of medium circulated by a peristaltic pump (not shown) at 5–10 ml min−1. The channel is surrounded by a second channel (lighter blue ink) that is connected to a vacuum; this vacuum channel seals the chip to the plastic sheet and prevents leaks. (B) A close up showing an individual embryo slot. White lines indicate the widths of the openings facing the channel; the difference leads to the pressure gradient that holds the embryos in place (direction indicated by arrow). (C) Image of a chip with embryos in each slot. Also visible are the attached tubes bringing medium to the chip. Image published in: Adams DS et al. (2016) Copyright © 2016. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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