Xenbase Image ID: 158893

Figure 1-Figure supplement 1: Characterization of mAb 2F4 to pcdh8l. A) Western blot from total membrane extracted from 10 embryos at gastrula stage (Stage 10) and early tailbud stage (Stage 20) detected using 815 mAb2F4. B-C) Cranial neural crest explants on fibronectin. Explants were left to migrate for 5 hours and fixed in 1XMBS, 3.7% formaldehyde for 1 hour, permeabilized for 30 min in 1XMBS containing 0.5% TritonX100. Staining with mAb 2F4 (B) clearly shows a membrane staining while DAPI stains the nuclei (C). D) Wholemount immunostaining of tailbud stage embryo fixed in DENTS (20% DMSO 80% Methanol) using mAb 2F4. The red arrows indicate the tip of the cranial neural crest segments. The staining is identical to the one obtained by in situ hybridization with the pcdh8l probe. E) Western blot on Hek293T cells extract transfected with the various Xenopus laevis protocadherin. The mAb 2F4 only recognizes pcdh8l (PCNS). Image published in: Khedgikar V et al. (2017) © 2017, Khedgikar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pcdh8l.L pcns X. laevis Throughout NF stage 24 cranial neural crest neural crest mandibular crest hyoid crest branchial crest anterior branchial crest posterior branchial crest somite Image source: Published Larger Image Printer Friendly View

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