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Figure 1-Figure supplement 2: adam13 overexpression increases pcdh8l fragments. Western blot with mAb 2F4 from glyctoproteins isolated from 20 embryos at stage 20. The intact full-length pcdh8l protein (PCNS) is indicated as well as 2 main fragments (arrowhead 1 and 2). All 3 forms are detected in uninjected embryos (UI) but the fragments are increased in embryos overexpressing adam13 (A13). The blot was reprobed with 15F, a polyclonal antibody directed against the adam13 cytoplasmic domain (35). The Pro (P) and Mature (M) forms of adam13 are indicated. Two exposures are provided to clearly detect the endogenous and overexpressed adam13 protein. Image published in: Khedgikar V et al. (2017) © 2017, Khedgikar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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