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Figure 2-Figure supplement 1: adam13 knockdown reduces pcdh8l expression in 848 craFigure 2-Figure supplement 1: adam13 knockdown reduces pcdh8l expression in cranial neural crest (CNC) cells. (A) Relative mRNA expression of pcdh8l normalized to GAPDH in isolated CNC explants. CNC explants (20) were dissected from either control embryos or embryos injected with MO13 (10 ng at 1-cell stage) at stage 17. (B) Western blot for pcdh8l and Rpn1 from CNC glycoproteins. Proteins were extracted from non-injected and MO13 injected CNC (20) and glycoproteins pulled down using Con-A agarose beads. Western blot shows that MO13 at 10 ng reduces pcdh8l expression compared to non-injected. Error bars represent standard error to the mean (Mean± S.E.M). One-way ANOVA was performed to determine statistical significance. Statistically significant at **p < 0.01.nial neural crest (CNC) cells. (A) Relative mRNA expression of pcdh8l normalized 849 to GAPDH in isolated CNC explants. CNC explants (20) were dissected from either 850 control embryos or embryos injected with MO13 (10 ng at 1-cell stage) at stage 17. (B) 851 Western blot for pcdh8l and Rpn1 from CNC glycoproteins. Proteins were extracted from 852 non-injected and MO13 injected CNC (20) and glycoproteins pulled down using Con-A853 agarose beads. Western blot shows that MO13 at 10 ng reduces pcdh8l expression 854 compared to non-injected. Error bars represent standard error to the mean (Mean± S.E.M). 855 One-way ANOVA was performed to determine statistical significance. Statistically 856 significant at **p < 0.01. Image published in: Khedgikar V et al. (2017) © 2017, Khedgikar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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