XB-IMG-158897
Xenbase Image ID: 158897
|
Figure 3: Full-length adam13 induces pcdh8l expression in naïve ectoderm (animal
858 cap cells). One-cell stage embryos were injected with mRNA encoding various forms of
859 adam13 (1 ng). Animal cap (AC) explants were dissected at stage 9 and grown in 0.5X
860 MBS until sibling embryos reached stage 18 to 20. mRNA and protein was extracted
from AC for analysis of 861 pcdh8l. (A) Quantitative real-time PCR from mRNA isolated
862 from 10 animal caps. The relative level of pcdh8l expression was normalized to GAPDH.
863 (B) Western blot for pcdh8l protein using mAb2F4. 30 AC were used to extract protein
864 from embryos injected with various adam13 constructs. Western blot shows induction of
865 pcdh8l by full-length adam13, whereas other constructs fails to induce pcdh8l expression.
866 adam13 (A13), non proteolytic adam13 (A13E/A), adam13 lacking a cytoplasmic domain
867 (Cyto), isolated adam13 cytoplasmic domain (GFP-C13). Error bars represent standard
868 error to the mean (Mean± S.E.M). One-way ANOVA was performed to determine
869 statistical significance. Statistically significant at ***p < 0.001 Image published in: Khedgikar V et al. (2017) © 2017, Khedgikar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |