XB-IMG-158898
Xenbase Image ID: 158898
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Figure 4. Adam13 knockdown reduces tfap2α expression. (A) Relative mRNA
expression of AP2α normalized to GAPDH (5 embryos). Real-time PCR analysis shows a
40% decrease in AP2 expression in response to adam13 KD (B) Representative images
of dorsal view of whole mount in situ hybridization with AP2. MO13 (5 ng) was
injected in one of the two blastomeres (star). The non-injected side serves as control.
adam13 KD reduces AP2 expression in 76% of the embryos (N=29). (C) Luciferase
activity of AP2α promoter in cranial neural crest cells (CNC). One-cell stage embryos
were injected with MO13 (10 ng). Control and KD embryos were further injected at the
8-cell stage with the AP2:luciferase reporter (100 pg) together with pRL-CMV (10 pg)
in one animal dorsal blastomere. CNC explants were dissected from stage 15-17 embryos
and individual explants were lysed to measure luciferase activity. The normalized values
from 10 individual explants (two biological replicates) were used for each measure. Error
bars represent standard error to the mean (Mean± S.E.M). One-way ANOVA was
performed to determine statistical significance. 883 Statistically significant at *p < 0.05, **p
< 0.01. Image published in: Khedgikar V et al. (2017) © 2017, Khedgikar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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