Xenbase Image ID: 158907

Figure 1. AT Mϕs in X. laevis.(A) Anatomy of the visceral fat depot. Scale bar, 1 cm. (B) Histologic assessment of the fat body by hematoxylin and eosin staining. Scale bar, 100 μm. (C) Enzyme histochemistry and ultrastructure of the fat body stroma, and quantification of ATMs. ACP- and peroxidase (POD)-positive cells in the fat body (top). Scale bar, 50 μm. TEM image of the fat body (bottom left). Arrowheads indicate ATMs (leukocytes with abundant endocytic vesicles). Scale bar, 1 μm. ATM content and percentage of phagocytosing (phag+) cells in the stroma (bottom right). The raw data set is available at the Flow Repository under accession number FR-FCM-ZYZF. (D) FACS analysis of SCs. For TEM images of the sorted RBCs, ATMs, and Lym, and for additional details of the FACS analysis, see Supplemental Fig. 1A–D. (E–G) Identification of phagocytosing cells in AT stroma. Isolated SCs were incubated in vitro with fluorescent latex beads for 4 h. (H) Time-lapse sequence of a phagocytosing ATM. The entire time lapse sequence is shown as Supplemental Video 1. Scale bar, 25 μm. (I) ATMs with ingested fluorescent latex beads (arrows). Scale bar, 15 μm. (J) TEM images of ATMs. Scale bar, 1 μm. Additional TEM images of ATMs are shown in Supplemental Fig. 1A. ac = adipocyte, fb = fat body; FSC = forward scatter, lp = lamellipodia, Lym = lymphocytes, nc = nucleus, ov, ovary; phag− = cells without phagocytosed beads, phag+ = cells with phagocytosed beads, SSC = side scatter, st = stroma, ves = blood vessel, vs = vesicles. Image published in: Hassnain Waqas SF et al. (2017) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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