Xenbase Image ID: 158908

Figure 2. Self-renewal of ATMs in X. laevis.(A) Isolated SCs were cultured in vitro and labeled with BrdU (indicated in red; n = 6). In the control experiment, cells were incubated with vehicle only (indicated in gray). Presence of BrdU+ cells was detected by FACS. The raw data set is available in the Flow Repository under accession number FR-FCM-ZYZG. (B) Characterization of BrdU+ cells with FACS. (C) Amount of BrdU+ ATMs after 2–4 h of incubation with BrdU. Histogram shows BrdU labeling after 4 h (n = 3). (D) SCs were cultured in vitro in soft agar. Colonies formed by SCs were detected after 24, 48, and 72 h. Arrow denotes cell-cell attachment site. Scale bar, 50 μm. (E) TEM images of SC colonies formed in soft agar. Representative images (E1-E3). Scale bar, 0.5 μm. cp = cytoplasm, FSC = forward scatter, Lym = lymphocytes, mt = mitochondria, nc = nucleus, SSC = side scatter. Image published in: Hassnain Waqas SF et al. (2017) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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