XB-IMG-158909
Xenbase Image ID: 158909
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Figure 3. Development of ATMs in X. laevis.(A) Relative transcription of the hematopoietic marker scl in SCs of the fat bodies and in various hematopoietic tissues (n = 6; *P < 0.05, Student’s 2-tailed, unpaired t test). (B) Analysis of scl:eGFP+ cells in adult fat bodies using FACS and histology (n = 3). Arrows show scl:eGFP+ cells. Scale bar, 50 μm. Additional details are shown in Supplemental Fig. 2. (C) Relative transcription of the Mϕ precursor marker, lurp, in SCs of the adult fat bodies and in various hematopoietic tissues (n = 6). (D) Analysis of lurp:eGFP+ cells in adult fat bodies using FACS and histology (n = 3). Arrows show lurp:eGFP+ cells. Scale bar, 50 μm. Additional details are shown in Supplemental Fig. 2. (E1) Presence of lurp:eGFP+ cells in developing fat bodies. Arrows show lurp:eGFP+ cells. Scale bars, 1 cm (in macroscopic images showing developing tadpoles) and 50 μm (for the histology images). (E2) FACS analysis of lurp:eGFP+ ATMs at various developmental stages (n = 5 each stage). The data set is available in the Flow Repository under accession number FR-FCM-ZYZE. (F) Anatomy of the fat body in A. mexicanum. Scale bar, 1 cm. (G) Hematoxylin and eosin (H&E)–stained section and ACP enzyme histochemistry of the fat body. Arrow shows an ACP+ cell. Scale bar, 50 μm. ac = adipocyte, fb = fat body, FSC = forward scatter, st = stroma, ov = ovary. Image published in: Hassnain Waqas SF et al. (2017) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |